Part:BBa_K4422007

8X-His tag

The production of recombinant proteins is essential for biotechnology. To be beneficial for research studies, these proteins generally need to be expressed and purified in large quantities. For years, the use of polyhistidine tags (His-tags), which typically contain six or eight consecutive histidine residues, has been a staple in the isolation of recombinant proteins by immobilized metal affinity chromatography (IMAC), where divalent cations (usually Ni2+ or Co2+) adhere to a solid matrix. Its use has been very beneficial to increase protein purity, thus, a tag introduced for purification may contain a specific epitope recognized by an antibody fragment immobilized on a chromatography column.

Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V., Offermann, L. R., Tang, C., Hurlburt, B. K., & Chruszcz, M. (2018). Impact of an N-terminal Polyhistidine Tag on Protein Thermal Stability. ACS omega, 3(1), 760–768. https://doi.org/10.1021/acsomega.7b01598

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]