Part:BBa_K4422005
Short Flexible Linker Gly-Gly-Ser-Gly (Pichia pastoris codon optimized)
As an indispensable component of recombinant fusion proteins, linkers have shown increasing importance in the construction of stable and bioactive fusion proteins. The successful construction of a recombinant fusion protein requires two indispensable elements: the component proteins and the linkers. The most widely used flexible linkers have sequences consisting primarily of stretches of Gly and Ser residues ("GS" linker), which provide good flexibility and solubility to the protein. These flexible linkers can increase the accessibility of an epitope to antibodies or improve protein folding.
Chen, X., Zaro, J. L., & Shen, W. C. (2013). Fusion protein linkers: property, design and functionality. Advanced drug delivery reviews, 65(10), 1357–1369. https://doi.org/10.1016/j.addr.2012.09.039
van Rosmalen, M., Krom, M., & Merkx, M. (2017). Tuning the Flexibility of Glycine-Serine Linkers To Allow Rational Design of Multidomain Proteins. Biochemistry, 56(50), 6565–6574. https://doi.org/10.1021/acs.biochem.7b00902
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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