Part:BBa_K4422002

GNA Lectin (Pichia pastoris codon optimized)

The plant lectin Galanthus nivalis agglutinin (GNA) significantly improves the oral efficacy of insecticidal peptides thanks to its ability to cross the intestinal epithelium, delivering the peptides to the insect's hemolymph. Furthermore, with the GNA lectin, we improve peptide stability and resistance against proteolysis.

Pyati, P., Fitches, E., & Gatehouse, J. A. (2014, August 1). Optimising expression of the recombinant fusion protein biopesticide ω-hexatoxin-Hv1a/GNA in Pichia pastoris: sequence modifications and a simple method for the generation of multi-copy strains. Journal of Industrial Microbiology and Biotechnology, 41(8), 1237–1247. https://doi.org/10.1007/s10295-014-1466-8.

Trung, N. P., Fitches, E., & Gatehouse, J. A. (2006). A fusion protein containing a lepidopteran-specific toxin from the South Indian red scorpion (Mesobuthus tamulus) and snowdrop lectin shows oral toxicity to target insects. BMC Biotechnology, 6, 1–12. https://doi.org/10.1186/1472-6750-6-18

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]