Coding

Part:BBa_K4419027

Designed by: Leonardo ken Okumura, Ryoko Enomoto   Group: iGEM22_TokyoTech   (2022-10-11)


EGFP
This sequence encodes EGFP, green flourescent protein

rank

Fig. 1.Confirmation of our restriction enzyme treatment from pBlueⅡScript(pBLⅡSK). Lane 1: pBLⅡSK (~3K bp) ; Lane 2: λHindⅢ ; Lene 3: pIRES2_EGFP (~5K bp); Lene4:pCAGGS (~4.8K bp);

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 679
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


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Categories
Parameters
None