DNA

Part:BBa_K4419023

Designed by: Leonardo ken Okumura, Ryoko Enomoto   Group: iGEM22_TokyoTech   (2022-10-02)


IRES
Internal Ribosome Entry Sites (IRES) are cis-acting RNA sequences able to mediate internal entry of the 40S ribosomal subunit on some eukaryotic and viral messenger RNAs upstream of a translation initiation codon.

rank

Fig. 1.Confirmation of our restriction enzyme treatment from pBlueⅡScript(pBLⅡSK). Lane 1: pBLⅡSK (~3K bp) ; Lane 2: λHindⅢ ; Lene 3: pIRES2_EGFP (~5K bp); Lene4:pCAGGS (~4.8K bp);

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None