Coding

Part:BBa_K4419022

Designed by: Leonardo ken Okumura, Ryoko Enomoto   Group: iGEM22_TokyoTech   (2022-10-02)


N-Cre(Mosquitoes)
This sequence encodes N-terminal region of Cre recombinase enzyme

rank

Fig. 1.Confirmation of our restriction enzyme treatment from pBlueScript. Lane 1: Blank ; Lane 2: pBL/HpaⅡ ; Lene 3: N-Cre(HUman) (~732 bp); Lene4:N-Cre(Mosquitoes) (~732 bp); Lene5: mCherry(Human) (~708 bp); Lene6: mCherry(Mosquitoes) (~708 bp)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 356
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 42
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None