Composite

Part:BBa_K4418008

Designed by: Jazz Ghataora   Group: iGEM22_UniofBath   (2022-10-10)


Xylose-inducible NOT gate for Bacillus subtilis

This composite part is a xylose inducible NOT gate which utilises the Bacillus subtilis PxylA promoter (BBa_K1351039) to drive transcription of the repressor SarA (BBa_K4418005), whose translation is initiated by a consensus B. subtilis RBS (BBa_K090505). Once expressed, the repressor SarA can repress its' target promoter, PsprC (BBa_K4418006) which we utilised to drive expression of a luciferase reporter. To prevent transcriptional read-through from PxylA, a strong terminator (BBa_K4418007) is used.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 382
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 382
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 382
    Illegal BamHI site found at 247
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 382
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 382
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None