Coding

Part:BBa_K4418005

Designed by: Jazz Ghataora   Group: iGEM22_UniofBath   (2022-10-10)


SarA, a repressor of sRNA expression in Staphylococcus aureus

SarA is a transcription repressor which represses the expression of two sRNAs in Staphylococcus aureus. One of the cognate promoters repressed by SarA is PsprC (BBa_K4418006), which we have used along with SarA to generate a functional and heterologous NOT gate in Bacillus subtilis.

https://doi.org/10.1093/nar/gkw777

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 106
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 106
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 106
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 106
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 106
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None