Part:BBa_K4418004
PpstS, a Bacillus subtilis native phosphate regulated promoter
Phosphate regulated promoter regulated by the PhoPR Two-Component System native to Bacillus subtilis.
The promoter PpstS drives expression of genes in the PstS-PstC-PstA-PstBA-PstBB operon in Bacillus subtilis used for phosphate importer, and is regulated by the PhoPR Two-Component System. In the presence of phosphate, the PpstS promoter is inactive due to the binding of the phosphorylated form of the response regulator, PhoP (PhoP-P). In the absence of phosphate, this phosphorylation does not occur and the PpstS promoter can become active and allow for the expression of high affinity phosphate binding proteins and transporters to import phosphate into the cell.
This promoter was used as part of a luciferase (luxABCDE) transcription fusion to generate a phosphate inducible biosensor. To conduct this work, we used MCSE medium in which the final concentration of phosphate was 0.01 mM (1:100 dilution of the 10X MOPS solution)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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