Regulatory

Part:BBa_K4418000

Designed by: Jazz Ghataora   Group: iGEM22_UniofBath   (2022-10-02)


PmaeN, a malate inducible promoter for Bacillus subtilis

PmaeN is a native B. subtilis malate inducible promoter regulated by the MalKR Two-Component System to enable the usage of malate as a carbon source. Previous study (see doi link) has shown this promoter is not induced by other carbon sources such as fumarate, succinate or even gluocse - thus not under the control of carbon catabolite repression.

Using a luxABCDE reporter system, we demonstrate this promoter shows an increase in RLU/OD activity following subjecting cells to increasing concentrations of malate (L-maleic acid) to the point of promoter saturation using 0.5 mM malate.

https://doi.org/10.1099/mic.0.26257-0

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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