Composite

Part:BBa_K4417022

Designed by: Jiaying Zou   Group: iGEM22_UCL   (2022-10-12)


CuO-RBS- CA22-rrnB T1 Terminator in pCT5c

Description

The codon optimized carbonic anhydrase part (BBa_K4417021) was cloned into pCT5c plasmid (BBa_K4417000) using restriction digest.

Figure 1:Codon optimized CA in pCT5c plasmid.

Cloning Strategy

NdeI and SacI sites were used to clone pCT5c plasmid (BBa_K4417000) with carbonic anhydrase gene ((BBa_K4417021)

Figure 2:CuO-RBS- CA22-rrnB T1 Terminator in pCT5c.

Due to time constraints, the cloning of this part into the plasmid has not been fully completed, but the design was feasible and rigorous.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1016
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 709
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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