Coding

Part:BBa_K4417020

Designed by: Jiaying Zou   Group: iGEM22_UCL   (2022-10-12)


CA22

Description

This part is a codon optimized sequence for an α-type carbonic anhydrase operon from Bacillus halodurans TSLV1. Carbonic anhydrase (CA) will facilitate the precipitation of CaCO3 by the interconversion of CO2 and HCO3-. We were inspired by SZU-China 2017 iGEM team (Part: BBa_K2232014) and would like to incorporate their carbonic anhydrase pathway into our urease pathway, making a complete cycle for biocementation. We also mutated three SapI sites in the original sequence to make it Type IIS compatible.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 827
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 520
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None