Plasmid_Backbone

Part:BBa_K4415000

Designed by: William Pihlainen-Bleecker   Group: iGEM22_McMaster_A   (2022-09-30)


f3-55nm-MCS chimeric phage

f3-55nm-MCS is a chimeric phage made using 2 fd-tet derived phage species; f3-55nm, and fMCS. Together, these phages allow us to create a cloning vector with the ability to display a protein on the pIII coat protein. SfiI sites flank a stuffer sequence that once removed, can be replaced with a suitable protein that will be expressed on the N-Terminus of the pIII proteins. The MCS contains multiple unique sites, allowing for insertion of any sequences of interest.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 745
    Illegal XbaI site found at 1431
    Illegal PstI site found at 8502
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 745
    Illegal PstI site found at 8502
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 745
    Illegal BglII site found at 8498
    Illegal BamHI site found at 5065
    Illegal XhoI site found at 8494
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 745
    Illegal XbaI site found at 1431
    Illegal PstI site found at 8502
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 745
    Illegal XbaI site found at 1431
    Illegal PstI site found at 8502
    Illegal AgeI site found at 309
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None