Coding

Part:BBa_K4410002

Designed by: Ruyi Shi   Group: iGEM22_Worldshaper-HZBIOX   (2022-09-19)


argR

This part is argR gene. argR gene is from the genome of E.coli Nissle 1917. It encodes argR protein, an arginine repressor causing negative inhibition of arginine biosynthesis in E coli bacteria. In our project, we knocked down argR gene by replacing argR gene with KanR gene, in order to prevent feedback inhibition of arginine synthesizing pathway from accumulated arginine.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 27
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 27
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 27
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 27
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None