Part:BBa_K4409004

trigger-RNA sensor of igRNA (hsa_circ_0001982)_Coupled with GAPDH as guide sequence

It is used as a sensing part of the RNA-interacting guide RNA (igRNA) in our CRISPR-Cas12 system to detect a unique sequence (trigger RNA) of the target circRNA hsa_circ_0001982. This part serves as a switch for the whole circuit. It contains three regions, VR1 region, loop region, and VR2 region. VR1 and VR2 regions complement to VR1’ and VR2’ regions (trigger RNA) of hsa_circ_0001982. Loop region is complementary to the guide of igRNA (GAPDH) (BBa_K4409017). When hsa_circ_0001982 is present in the system, VR1 and VR2 regions combine with VR1’ and VR2’ sequences. Thus, loop region unblocks the guide of igRNA (GAPDH) (BBa_K4409017) which can then bind with dsDNA (GAPDH) (BBa_K4409006) and activate Cas enzyme to cleave DNAs.

【Graph 1】The made up of igRNA

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]