DNA

Part:BBa_K4409001

Designed by: Kexin Fei   Group: iGEM22_Worldshaper-HZ   (2022-09-06)


guide of igRNA (hsa_circ_0001785)

BBa_K4409002 serves as a guide sequence of the RNA-interacting guide RNA (igRNA) used for a CRISPR-Cas12 system in our project. It can combine with a substrate double strand DNA (dsDNA) of (BBa_K4409007) with a PAM sequence for Cas12a protein. After the binding, the igRNA activates the Cas12a enzyme to cleave the DNA. To form an igRNA, this part can be coupled with our trigger-RNA sensor (BBa_K4409005) which contains a loop sequence complementary to it, so that the loop sequence can block this part when no trigger is in the system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None