Plasmid

Part:BBa_K4404009

Designed by: Julia Fricke   Group: iGEM22_Goettingen   (2022-10-09)


acsB1 from A. woodii under the control of a PbgaL promoter from C. perfringens

This composite part consists of plasmid backbone pMTL83151 (BBa_K4404007) and acsB1 (BBa_K4404000) which is under the control of the PbgaL promoter (BBa_K2992023). The promoter from C. perfringens functions in Gram-negative (E.coli) and in Gram-positive (Clostridium saccharoperbutylacetonicum) bacteria and is inducible by lactose. The insert acsB1 codes for the acetyl-CoA synthase subunit (ACS) of the CODH/ACS-complex which catalyzes the formation of acetyl-CoA from CO and [CH3]-Co-FeS-P. acsB was inserted into the backbone pMTL83151 to complement the Wood-Ljungdahl metabolic pathway for CO2 assimilation.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1599
    Illegal EcoRI site found at 1944
    Illegal EcoRI site found at 2232
    Illegal EcoRI site found at 2679
    Illegal EcoRI site found at 6154
    Illegal XbaI site found at 6181
    Illegal PstI site found at 6044
    Illegal PstI site found at 6220
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1599
    Illegal EcoRI site found at 1944
    Illegal EcoRI site found at 2232
    Illegal EcoRI site found at 2679
    Illegal EcoRI site found at 6154
    Illegal NheI site found at 2286
    Illegal NheI site found at 6399
    Illegal PstI site found at 6044
    Illegal PstI site found at 6220
    Illegal NotI site found at 6123
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1599
    Illegal EcoRI site found at 1944
    Illegal EcoRI site found at 2232
    Illegal EcoRI site found at 2679
    Illegal EcoRI site found at 6154
    Illegal BglII site found at 6208
    Illegal BamHI site found at 2270
    Illegal BamHI site found at 6175
    Illegal XhoI site found at 105
    Illegal XhoI site found at 6212
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1599
    Illegal EcoRI site found at 1944
    Illegal EcoRI site found at 2232
    Illegal EcoRI site found at 2679
    Illegal EcoRI site found at 6154
    Illegal XbaI site found at 6181
    Illegal PstI site found at 6044
    Illegal PstI site found at 6220
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1599
    Illegal EcoRI site found at 1944
    Illegal EcoRI site found at 2232
    Illegal EcoRI site found at 2679
    Illegal EcoRI site found at 6154
    Illegal XbaI site found at 6181
    Illegal PstI site found at 6044
    Illegal PstI site found at 6220
    Illegal NgoMIV site found at 3593
    Illegal NgoMIV site found at 5500
    Illegal NgoMIV site found at 5510
    Illegal NgoMIV site found at 5636
    Illegal AgeI site found at 215
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 5252
    Illegal SapI.rc site found at 5607
    Illegal SapI.rc site found at 5978


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