Plasmid

Part:BBa_K4404008

Designed by: Julia Fricke   Group: iGEM22_Goettingen   (2022-10-09)


acsB2 from C. ljungdahlii under the control of a promoter from the C. acetobutylicum thiolase gene

This composite part consists of the plasmid backbone pMTL83151 (BBa_K4404007) and acsB2 (BBa_K4404001) which is under the control of the thiolase promoter (BBa_K2715019). The constitutive promoter from C. acetobutylicum thiolase gene functions in Gram-negative (E.coli) and in Gram-positive (Clostridium saccharoperbutylacetonicum) bacteria. The insert acsB2 codes for the acetyl-CoA synthase subunit (ACS) of the CODH/ACS-complex which catalyzes the formation of acetyl-CoA from CO and [CH3]-Co-FeS-P. acsB was inserted into the backbone pMTL83151 to complement of the Wood-Ljungdahl metabolic pathway for CO2 assimilation.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 428
    Illegal EcoRI site found at 3903
    Illegal XbaI site found at 3930
    Illegal SpeI site found at 6223
    Illegal PstI site found at 3793
    Illegal PstI site found at 3969
    Illegal PstI site found at 4988
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 428
    Illegal EcoRI site found at 3903
    Illegal NheI site found at 4148
    Illegal NheI site found at 6676
    Illegal SpeI site found at 6223
    Illegal PstI site found at 3793
    Illegal PstI site found at 3969
    Illegal PstI site found at 4988
    Illegal NotI site found at 3872
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 428
    Illegal EcoRI site found at 3903
    Illegal BglII site found at 3957
    Illegal BamHI site found at 3924
    Illegal BamHI site found at 6660
    Illegal XhoI site found at 3961
    Illegal XhoI site found at 4543
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 428
    Illegal EcoRI site found at 3903
    Illegal XbaI site found at 3930
    Illegal SpeI site found at 6223
    Illegal PstI site found at 3793
    Illegal PstI site found at 3969
    Illegal PstI site found at 4988
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 428
    Illegal EcoRI site found at 3903
    Illegal XbaI site found at 3930
    Illegal SpeI site found at 6223
    Illegal PstI site found at 3793
    Illegal PstI site found at 3969
    Illegal PstI site found at 4988
    Illegal NgoMIV site found at 1342
    Illegal NgoMIV site found at 3249
    Illegal NgoMIV site found at 3259
    Illegal NgoMIV site found at 3385
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3001
    Illegal SapI.rc site found at 3356
    Illegal SapI.rc site found at 3727


[edit]
Categories
Parameters
None