Coding

Part:BBa_K4404005

Designed by: Julia Fricke   Group: iGEM22_Goettingen   (2022-10-09)


metV and metF from A. woodii

This is a fragment with a length of 1.573 kb, which was synthesised by de-novo-synthesis with the genomic background of Acetobacerium woodii. It consists of the genes metV and metF which encode two subunits of methylene-THF reductase. All these genes are parts of the Wood-Ljungdahl pathway of acetogenic bacteria. The gene coding fragment is flanked by specific restriction sites and overhangs. Upstream it is flanked by a Pac1 restriction site and a 5 base pairs overhang. Downstream it is flanked by a Nhe1 restriction site and a 5 base pairs overhang.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1513
    Illegal PstI site found at 709
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1513
    Illegal NheI site found at 1563
    Illegal PstI site found at 709
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1513
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1513
    Illegal PstI site found at 709
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1513
    Illegal PstI site found at 709
    Illegal AgeI site found at 1019
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None