![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4404005
Designed by: Julia Fricke Group: iGEM22_Goettingen (2022-10-09)
metV and metF from A. woodii
This is a fragment with a length of 1.573 kb, which was synthesised by de-novo-synthesis with the genomic background of Acetobacerium woodii. It consists of the genes metV and metF which encode two subunits of methylene-THF reductase. All these genes are parts of the Wood-Ljungdahl pathway of acetogenic bacteria. The gene coding fragment is flanked by specific restriction sites and overhangs. Upstream it is flanked by a Pac1 restriction site and a 5 base pairs overhang. Downstream it is flanked by a Nhe1 restriction site and a 5 base pairs overhang.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1513
Illegal PstI site found at 709 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1513
Illegal NheI site found at 1563
Illegal PstI site found at 709 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1513
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1513
Illegal PstI site found at 709 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1513
Illegal PstI site found at 709
Illegal AgeI site found at 1019 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |