Coding

Part:BBa_K4404004

Designed by: Julia Fricke   Group: iGEM22_Goettingen   (2022-10-09)


cooC1, acsV, AWO_c10690 and AWO_c10700 from A. woodii

This is a fragment with a length of 4.12 kb, which was synthesised by de-novo-synthesis with the genomic background of Acetobacerium woodii. It consists of the genes cooC1, acsV, AWO_c10690 and AWO_c10700. cooC1 codes for an accessory protein of the Ni2+ insertion. acsV regenerates the corrinoid iron sulfur protein. AWO_c10690 and AWO_c10700 are putative proteins. All these genes are parts of the Wood-Ljungdahl pathway of acetogenic bacteria. The gene coding fragment is flanked by specific restriction sites and overhangs. Upstream it is flanked by a Sal1 restriction site and a 5 base pairs overhang. Downstream it is flanked by a Pac1 restriction site and a 5 base pairs overhang.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1561
    Illegal EcoRI site found at 1887
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1561
    Illegal EcoRI site found at 1887
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1561
    Illegal EcoRI site found at 1887
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1561
    Illegal EcoRI site found at 1887
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1561
    Illegal EcoRI site found at 1887
    Illegal AgeI site found at 66
    Illegal AgeI site found at 1978
    Illegal AgeI site found at 2575
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2942


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Categories
Parameters
None