Part:BBa_K4404002
acsA, cooC2 and acsB1 from A. woodii with a Sal1 restriction site
This is a fragment with a length of 4.942 kb, which was amplified from the genomic DNA of Acetobacerium woodii. It consists of the genes ascB1, cooC2 and acsA. cooC2 and acsA are located immediately upstream of acsB1. acsB1 codes for the acetyl-CoA synthase subunit (ACS) of the CODH/ACS-complex which catalyzes the formation of acetyl-CoA from CO and [CH3]-Co-FeS-P. cooC2 codes for the putative CODH nickel-insertion accessory protein. acsA partially codes for the carbon monoxide dehydrogenase subunit of the CODH/ACS-complex, which catalyzes the reduction of CO2 to CO. All these genes are parts of the Wood-Ljungdahl pathway of acetogenic bacteria. The gene coding fragment is flanked by specific restriction sites and overhangs. Upstream it is flanked by a Sal1 restriction site and a 5 base pairs overhang. Downstream it is flanked by a Nhe1 restriction site and a 5 base pairs overhang.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 692
Illegal EcoRI site found at 4268
Illegal EcoRI site found at 4613
Illegal EcoRI site found at 4901 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 692
Illegal EcoRI site found at 4268
Illegal EcoRI site found at 4613
Illegal EcoRI site found at 4901
Illegal NheI site found at 4955 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 692
Illegal EcoRI site found at 4268
Illegal EcoRI site found at 4613
Illegal EcoRI site found at 4901
Illegal BamHI site found at 4939
Illegal XhoI site found at 303 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 692
Illegal EcoRI site found at 4268
Illegal EcoRI site found at 4613
Illegal EcoRI site found at 4901 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 692
Illegal EcoRI site found at 4268
Illegal EcoRI site found at 4613
Illegal EcoRI site found at 4901
Illegal AgeI site found at 2884 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 2137
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