Composite

Part:BBa_K4398011

Designed by: Reinis Muiznieks   Group: iGEM22_Latvia-Riga   (2022-10-11)


Kanamycin resistance transcriptional unit

A transcriptional unit created for Rhodotorula toruloides with the RFC1000 standard which contains a strong constitutive promoter, kanamycin resistance coding sequence and a terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2434
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2434
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 272
    Illegal BglII site found at 487
    Illegal XhoI site found at 960
    Illegal XhoI site found at 1044
    Illegal XhoI site found at 1316
    Illegal XhoI site found at 1827
    Illegal XhoI site found at 2298
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2434
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2434
    Illegal AgeI site found at 1124
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None