Composite

Part:BBa_K4398009

Designed by: Reinis Muiznieks   Group: iGEM22_Latvia-Riga   (2022-10-11)


GLT2 transcriptional unit

A transcriptional unit created for Rhodotorula toruloides with the RFC1000 standard which contains a strong constitutive promoter, crocetin glucosyltransferase 2 coding sequence and a terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3001
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3001
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 272
    Illegal BglII site found at 487
    Illegal BglII site found at 2866
    Illegal XhoI site found at 960
    Illegal XhoI site found at 1044
    Illegal XhoI site found at 1316
    Illegal XhoI site found at 2130
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3001
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3001
    Illegal AgeI site found at 1124
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None