Composite

Part:BBa_K4398008

Designed by: Reinis Muiznieks   Group: iGEM22_Latvia-Riga   (2022-10-11)


Beta carotene hydroxylase transcriptional unit

A transcriptional unit created for Rhodotorula toruloides with the RFC1000 standard which contains a strong constitutive promoter, beta carotene hydroxylase coding sequence and a terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2536
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2536
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 272
    Illegal BglII site found at 487
    Illegal BglII site found at 1510
    Illegal XhoI site found at 960
    Illegal XhoI site found at 1044
    Illegal XhoI site found at 1316
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2536
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2536
    Illegal AgeI site found at 1124
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None