Composite

Part:BBa_K4398007

Designed by: Reinis Muiznieks   Group: iGEM22_Latvia-Riga   (2022-10-11)


ZCD transcriptional unit

A transcriptional unit created for Rhodotorula toruloides with the RFC1000 standard which contains a strong constitutive promoter, zeaxanthin 7,8(7',8')-cleavage dioxygenase coding sequence and a terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2728
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2728
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 272
    Illegal BglII site found at 487
    Illegal XhoI site found at 960
    Illegal XhoI site found at 1044
    Illegal XhoI site found at 1316
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2728
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2728
    Illegal NgoMIV site found at 1892
    Illegal AgeI site found at 1124
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None