Coding

Part:BBa_K4398006

Designed by: Reinis Muiznieks   Group: iGEM22_Latvia-Riga   (2022-10-11)


Kanamycin resistance for Rhodotorula toruloides

Kanamycin resistace coding sequence which has been codon-optimized for Rhodotorula toruloides using DNA Chisel python library. The part has been created for the use with the RFC1000 standard, therefore lacks the ATG start codon, which is introduced within the fusion sites after GGA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 325
    Illegal XhoI site found at 796
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None