Coding

Part:BBa_K4398005

Designed by: Reinis Muiznieks   Group: iGEM22_Latvia-Riga   (2022-10-11)


Hygromycin resistance for Rhodotorula toruloides

Hygromycin resistace coding sequence which has been codon-optimized for Rhodotorula toruloides using DNA Chisel python library. The part has been created for the use with the RFC1000 standard, therefore lacks the ATG start codon, which is introduced within the fusion sites after GGA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 104
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 904
    Illegal AgeI site found at 342
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None