Part:BBa_K4397007

IT2: Tau Aptamer

Type: DNA

Target: Tau 441

Binding Affinity: 5.5 nM ± 1.1 nM (ELONA)

Sequence IT2 demonstrates a unique binding profile on three of the peptides (T231, T231P, and S202) [1]. IT2 was at first truncated to IT2a based on an evident stem–loop motif observed in the predicted secondary structure, and IT2a demonstrated binding ability equal to IT2 for targets T231, T231P, and S202 [1]. However, IT2b failed to maintain the properties of the original aptamer. Sequence IT2c presented binding ability similar to IT2a, but a gradual loss of binding ability was observed by further truncating the stem of IT2c into IT2d and IT2e, suggesting the importance of a stable stem for binding of aptamer IT2c to its targets [1].

[1] Teng, I. T., Li, X., Yadikar, H. A., Yang, Z., Li, L., Lyu, Y., ... & Tan, W. (2018). Identification and characterization of DNA aptamers specific for phosphorylation epitopes of tau protein. Journal of the American Chemical Society, 140(43), 14314-14323.