Part:BBa_K4397006
Tau Aptamer 3146
Tau Aptamer: 3146
Target: Tau 441, Tau 381, Tau 352, and Tau 383
Binding Affinity: 13 nM, 116 nM, 84 nM, and 49 nM (SPR)
Usage and Biology
DNA aptamer 3146 is obtained from a 5 × 1012 DNA oligonucleotide library in only three rounds that can be performed within one day [1]. Target of 3146 aptamer is 2N4R-Tau, with selectivity for a monomer and with the Kd value of 13 nM (SPR) [2]. Aptamer 3146 bound tau isoforms in the following order: 2N4R (KD = 13 nM) > 0N4R (49 nM) > 0N3R (84 nM) > 1N3R (116 nM) [2].
[1]Storhoff, J. J., Elghanian, R., Mucic, R. C., Mirkin, C. A., & Letsinger, R. L. (1998). One-pot colorimetric differentiation of polynucleotides with single base imperfections using gold nanoparticle probes. Journal of the American Chemical Society, 120(9), 1959-1964.
[2] Murakami, K., Obata, Y., Sekikawa, A., Ueda, H., Izuo, N., Awano, T., ... & Irie, K. (2020). An RNA aptamer with potent affinity for a toxic dimer of amyloid β42 has potential utility for histochemical studies of Alzheimer's disease. Journal of Biological Chemistry, 295(15), 4870-4880.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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