Part:BBa_K4397003
RNV95
Amyloid Beta Aptamer: RNV95
Target: Amyloid-Beta 40 monomer
Binding Affinity: 50 - 400 nM (SPR)
Usage and Biology
RNV95 is a DNA aptamer with ability to detect tetrameric/pentameric low-molecular-weight Aβ aggregates [1]. During Western Blot analysis, both RNV95 and anti-Aβ antibodies detect a high-molecular-weight aggregate between 75 and 100 kDa in low yields, RNV95 shows higher intensities in detecting Aβ band than the anti-Aβ antibody [1]. Moreover, RNV95 does not show the ∼15 kDa band corresponding tetrameric/pentameric Aβ aggregates in healthy control brain tissue samples which is indicative of the binding specificity of RNV95 to AD [1]. Therefore, according to this research RNV95 could be a better alternative to the characterized monoclonal anti-Aβ antibody to detect low-molecular-weight aggregates of Aβ in AD brain tissue by western blot [1].
[1] Chakravarthy, M., AlShamaileh, H., Huang, H., Tannenberg, R. K., Chen, S., Worrall, S., ... & Veedu, R. N. (2018). Development of DNA aptamers targeting low-molecular-weight amyloid-β peptide aggregates in vitro. Chemical Communications, 54(36), 4593-4596.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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