Coding

Part:BBa_K4392011

Designed by: Atharva Valanju   Group: iGEM22_IISER-Pune-India   (2022-10-11)


exoB2 gene from Azospirillum brasilense

exoB and exoB2 being UDP-glucose 4-epimerases, are homodimeric epimerases involved in the final step of galactose metabolism, also known as the Leroir pathway. They are involved in the reversible conversion of UDP-galactose to UDP-glucose. They require the cofactor NAD+ for their functional activity. Their upregulation has been shown to increase the production of exopolysaccharides though the exact mechanism is not known.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 629
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 37
    Illegal NgoMIV site found at 526
    Illegal NgoMIV site found at 723
    Illegal NgoMIV site found at 850
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 406


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Parameters
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