Part:BBa_K4392006

ACC deaminase gene from Azospirillum lipoferum 4B

This gene often denoted as the acdS gene, codes for the 1-aminocyclopropane-1-carboxylate(ACC) deaminase enzyme which is involved in the deamination of the ethylene precursor - ACC into ammonia and alpha ketobutyrate.The deamination of plant-synthesized ACC diminishes the amount of ACC available for the production of the phytohormone ethylene. During abiotic stress conditions, the excessive ethylene produced by the plant is deleterious and leads to destruction of macromolecules and inhibition of photochemical processes in plants. This gene is naturally found on a 750kB (mega)plasmid in Azospirillum lipoferum 4B, and it was observed that this (mega)plasmid is lost in phenotypic variation.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 98
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 451
  Illegal NgoMIV site found at 478
  Illegal NgoMIV site found at 610
  Illegal NgoMIV site found at 975
  Illegal AgeI site found at 595
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 820