Plasmid
Part:BBa_K4392004
Designed by: Vikram Venugopal Group: iGEM22_IISER-Pune-India (2022-10-01)
pUC57-acdS
This part is the pUC57 plasmid, into which the ACC deaminase gene acdS from Pseudomonas fluorescens has been inserted. The acdS gene is flanked by a BioBrick prefix and suffix. This plasmid was used to obtain the acdS gene through PCR amplification of the acdS gene.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 396
Illegal suffix found in sequence at 1433 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 396
Illegal SpeI site found at 1434
Illegal PstI site found at 1448
Illegal NotI site found at 402
Illegal NotI site found at 1441 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 396
Illegal BamHI site found at 1285
Illegal XhoI site found at 602 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 396
Illegal suffix found in sequence at 1434 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 396
Illegal XbaI site found at 411
Illegal SpeI site found at 1434
Illegal PstI site found at 1448
Illegal NgoMIV site found at 1390 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 625
Illegal SapI site found at 1684
[edit]
Categories
Parameters
None |