Plasmid

Part:BBa_K4392004

Designed by: Vikram Venugopal   Group: iGEM22_IISER-Pune-India   (2022-10-01)


pUC57-acdS

This part is the pUC57 plasmid, into which the ACC deaminase gene acdS from Pseudomonas fluorescens has been inserted. The acdS gene is flanked by a BioBrick prefix and suffix. This plasmid was used to obtain the acdS gene through PCR amplification of the acdS gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 396
    Illegal suffix found in sequence at 1433
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 396
    Illegal SpeI site found at 1434
    Illegal PstI site found at 1448
    Illegal NotI site found at 402
    Illegal NotI site found at 1441
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 396
    Illegal BamHI site found at 1285
    Illegal XhoI site found at 602
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 396
    Illegal suffix found in sequence at 1434
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 396
    Illegal XbaI site found at 411
    Illegal SpeI site found at 1434
    Illegal PstI site found at 1448
    Illegal NgoMIV site found at 1390
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 625
    Illegal SapI site found at 1684


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Parameters
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