Composite

Part:BBa_K4392003

Designed by: Vikram Venugopal   Group: iGEM22_IISER-Pune-India   (2022-10-01)


T7 overexpression part for acdS

This part consists of the T7 promoter, the T7 RBS, the 1-aminocyclopropane-1-carboxylic acid deaminase gene (ACC deaminase/acdS), a 6x His tag fusion, and the T7 terminator. It overexpresses the acdS gene in a T7 overexpression system such as E.coli BL21(DE3). It can be used to purify the ACC deaminase enzyme.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 39
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 950
    Illegal BamHI site found at 1098
    Illegal XhoI site found at 267
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 39
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 39
    Illegal NgoMIV site found at 1055
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 290
    Illegal BsaI.rc site found at 20


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Categories
Parameters
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