Composite

Part:BBa_K4390106

Designed by: Maarten van den Ancker   Group: iGEM22_Edinburgh-UHAS_Ghana   (2022-09-21)


Constitutive iSpinach

This part was used by Edinburgh-UHAS_Ghana team of 2022 to test cell-free transcription protocols. The construct contains an F30 flanked iSpinach aptamer (BBa_K4390094) under a strong T7 RNA promoter (BBa_I712074). When used in a cell-free transcription system, the construct is trancscribed by a T7 RNA polymerase constitutively, causing fluorescence when the RNA aptamer is in the presence of DFHBI or DFHO.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None