Composite

Part:BBa_K4388010

Designed by: Venkata Kalyan Ghadiyaram   Group: iGEM22_KCL_UK   (2022-10-03)


Four transcriptional units expressing mutated genes for purpose of pterostilbene production

Synthetic operon comprised of four transcriptional units assembled via type IIS JUMP assembly for the purposes of expressing mutated genes that catalyze the production of pterostilbene.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 4098
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2115
    Illegal BglII site found at 3549
    Illegal BamHI site found at 5607
    Illegal BamHI site found at 5857
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None