Composite

Part:BBa_K4388006

Designed by: Tomas Lopes   Group: iGEM22_KCL_UK   (2022-10-09)


Transcriptional Unit 2 in Synthetic Operon for Pterostilbene Production

This composite part is the second transcriptional unit (TU) of the 4-gene synthetic operon constructed for pterostilbene production from L-tyrosine as precursor. This part includes a linker (replacing promoter) and an RBS (BBa_K4388011), an Arabidopsis thaliana 4-coumarate:CoA ligase mutant (BBa_K4388002), and a linker sequence that replaces the transcriptional terminator (BBa_K4388012). This composite part was assembled via JUMP type IIS assembly whereby the three above-mentioned basic parts were ligated into the pJUMP29-1B vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1277
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None