Composite

Part:BBa_K4383062

Designed by: Junxiao Zhang   Group: iGEM22_SCU-China   (2022-10-12)


J23101-EryO-B0034-TMADH-his operon terminator

replace the sfGFP region with TMADH gene as the plasmid BBa_K4383056,prove induction system functions successfully


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 536
    Illegal PstI site found at 331
    Illegal PstI site found at 1930
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 536
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 331
    Illegal PstI site found at 1930
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 536
    Illegal XhoI site found at 1865
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 536
    Illegal PstI site found at 331
    Illegal PstI site found at 1930
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 536
    Illegal PstI site found at 331
    Illegal PstI site found at 1930
    Illegal AgeI site found at 1027
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None