Composite
pqq

Part:BBa_K4383046

Designed by: Junxiao Zhang   Group: iGEM22_SCU-China   (2022-10-11)


J23101-B0034-pqqA-pqqB-pqqC-pqqD-pqqE-pqqF

pqq synthetic gene cluster under the control of J23101

Pqq.jpeg

figure1.pqq synthetic pathway

Dh pqq.png

figure2.pqq production in E. coli DH5a

Pqq BL21.png

figure3.pqq production in E. coli BL21

ECN pqq.png

figure4.pqq production in E. coli ECN

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 872
    Illegal PstI site found at 1537
    Illegal PstI site found at 1745
    Illegal PstI site found at 1805
    Illegal PstI site found at 1915
    Illegal PstI site found at 2221
    Illegal PstI site found at 3213
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 872
    Illegal PstI site found at 1537
    Illegal PstI site found at 1745
    Illegal PstI site found at 1805
    Illegal PstI site found at 1915
    Illegal PstI site found at 2221
    Illegal PstI site found at 3213
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2370
    Illegal BamHI site found at 1353
    Illegal XhoI site found at 3190
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 872
    Illegal PstI site found at 1537
    Illegal PstI site found at 1745
    Illegal PstI site found at 1805
    Illegal PstI site found at 1915
    Illegal PstI site found at 2221
    Illegal PstI site found at 3213
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 872
    Illegal PstI site found at 1537
    Illegal PstI site found at 1745
    Illegal PstI site found at 1805
    Illegal PstI site found at 1915
    Illegal PstI site found at 2221
    Illegal PstI site found at 3213
    Illegal NgoMIV site found at 215
    Illegal NgoMIV site found at 404
    Illegal NgoMIV site found at 881
    Illegal NgoMIV site found at 967
    Illegal NgoMIV site found at 2015
    Illegal NgoMIV site found at 2068
    Illegal NgoMIV site found at 2539
    Illegal AgeI site found at 1260
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4008
    Illegal BsaI.rc site found at 1981
    Illegal SapI.rc site found at 2866
    Illegal SapI.rc site found at 4624


[edit]
Categories
Parameters
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