Coding
PqqF

Part:BBa_K4383024

Designed by: Junxiao Zhang   Group: iGEM22_SCU-China   (2022-09-30)


PqqF

catalyzing the hydrolysis of multiple peptide bonds in PqqA

Pqq.jpeg

figure1.pqq synthetic pathway

Dh pqq.png

figure2.pqq production in E. coli DH5a

Pqq BL21.png

figure3.pqq production in E. coli BL21

ECN pqq.png

figure4.pqq production in E. coli ECN


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 40
    Illegal PstI site found at 328
    Illegal PstI site found at 520
    Illegal PstI site found at 583
    Illegal PstI site found at 703
    Illegal PstI site found at 955
    Illegal PstI site found at 1117
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 40
    Illegal PstI site found at 328
    Illegal PstI site found at 520
    Illegal PstI site found at 583
    Illegal PstI site found at 703
    Illegal PstI site found at 955
    Illegal PstI site found at 1117
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 40
    Illegal PstI site found at 328
    Illegal PstI site found at 520
    Illegal PstI site found at 583
    Illegal PstI site found at 703
    Illegal PstI site found at 955
    Illegal PstI site found at 1117
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 40
    Illegal PstI site found at 328
    Illegal PstI site found at 520
    Illegal PstI site found at 583
    Illegal PstI site found at 703
    Illegal PstI site found at 955
    Illegal PstI site found at 1117
    Illegal NgoMIV site found at 103
    Illegal NgoMIV site found at 666
    Illegal NgoMIV site found at 836
    Illegal NgoMIV site found at 1675
    Illegal NgoMIV site found at 2243
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 714
    Illegal SapI.rc site found at 1330


[edit]
Categories
Parameters
None