Coding
PqqE

Part:BBa_K4383023

Designed by: Junxiao Zhang   Group: iGEM22_SCU-China   (2022-09-30)


PqqE

contains a conserved CxxxCxxC, converts SAM to 50-deoxyadenosine Pqq.jpeg

figure1.pqq synthetic pathway

Dh pqq.png

figure2.pqq production in E. coli DH5a

Pqq BL21.png

figure3.pqq production in E. coli BL21

ECN pqq.png

figure4.pqq production in E. coli ECN


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 70
    Illegal PstI site found at 1062
    Illegal PstI site found at 1087
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 70
    Illegal PstI site found at 1062
    Illegal PstI site found at 1087
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 219
    Illegal XhoI site found at 1039
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 70
    Illegal PstI site found at 1062
    Illegal PstI site found at 1087
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 70
    Illegal PstI site found at 1062
    Illegal PstI site found at 1087
    Illegal NgoMIV site found at 388
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 715


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Categories
Parameters
None