Coding
PqqE
Part:BBa_K4383023
Designed by: Junxiao Zhang Group: iGEM22_SCU-China (2022-09-30)
PqqE
contains a conserved CxxxCxxC, converts SAM to 50-deoxyadenosine
figure1.pqq synthetic pathway
figure2.pqq production in E. coli DH5a
figure3.pqq production in E. coli BL21
figure4.pqq production in E. coli ECN
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 70
Illegal PstI site found at 1062
Illegal PstI site found at 1087 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 70
Illegal PstI site found at 1062
Illegal PstI site found at 1087 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 219
Illegal XhoI site found at 1039 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 70
Illegal PstI site found at 1062
Illegal PstI site found at 1087 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 70
Illegal PstI site found at 1062
Illegal PstI site found at 1087
Illegal NgoMIV site found at 388 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 715
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Categories
Parameters
None |