Coding
PqqD

Part:BBa_K4383022

Designed by: Junxiao Zhang   Group: iGEM22_SCU-China   (2022-09-30)


PqqD

bound to pqqA to help pqqA to be recognized by pqqE

Pqq.jpeg

figure1.pqq synthetic pathway

Dh pqq.png

figure2.pqq production in E. coli DH5a

Pqq BL21.png

figure3.pqq production in E. coli BL21

ECN pqq.png

figure4.pqq production in E. coli ECN

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 43
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 43
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 43
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 43
    Illegal NgoMIV site found at 143
    Illegal NgoMIV site found at 196
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 109


[edit]
Categories
Parameters
None