Coding
PqqC

Part:BBa_K4383021

Designed by: Junxiao Zhang   Group: iGEM22_SCU-China   (2022-09-30)


PqqC

the final step of the biosynthesis of PQQ,involves a ring closure and eight-electron oxidation of AHQQ (3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid)

Pqq.jpeg

figure1.pqq synthetic pathway

Dh pqq.png

figure2.pqq production in E. coli DH5a

Pqq BL21.png

figure3.pqq production in E. coli BL21

ECN pqq.png

figure4.pqq production in E. coli ECN

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 423
    Illegal PstI site found at 631
    Illegal PstI site found at 691
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 423
    Illegal PstI site found at 631
    Illegal PstI site found at 691
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 239
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 423
    Illegal PstI site found at 631
    Illegal PstI site found at 691
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 423
    Illegal PstI site found at 631
    Illegal PstI site found at 691
    Illegal AgeI site found at 146
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None