Coding
SldH

Part:BBa_K4383016

Designed by: Junxiao Zhang   Group: iGEM22_SCU-China   (2022-09-30)


SldH(polyol dehydrogenase)

An enzymes function as the main polyol dehydrogenase in Gluconobacter suboxydans IFO 3255. It contains two subunits sldA and sldB. sldA is the main functional subunit, and the sldB has a chaperone-like function to process the SLDH polypeptide into a mature and active form.Active site of SDH is oriented toward theperiplasm. This enzyme could catalyze the erythritol to the L-erythrulose with the coenzyme PQQ.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1218
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1218
    Illegal NheI site found at 2439
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1218
    Illegal BglII site found at 1674
    Illegal BamHI site found at 1694
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1218
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1218
    Illegal NgoMIV site found at 809
    Illegal NgoMIV site found at 2214
    Illegal AgeI site found at 238
    Illegal AgeI site found at 1406
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1182
    Illegal BsaI.rc site found at 1735
    Illegal SapI.rc site found at 745


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Categories
Parameters
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