Translational_Unit

Part:BBa_K4382014

Designed by: UZAIR MOTIWALA   Group: iGEM22_IISER_Bhopal   (2022-10-12)


Pectinase gene construct

The gene construct contains of 3 pectinase genes,a strong RBS(BBa_K4382009),and a signal peptide(BBa_K1074014).

Usage and Biology

The RBS is used to produce high levels of proteins and the signal peptide is used to secrete the enzymes.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2752
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2752
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 690
    Illegal BamHI site found at 2896
    Illegal BamHI site found at 3784
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2752
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2752
    Illegal NgoMIV site found at 3047
    Illegal AgeI site found at 2903
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 794
    Illegal BsaI site found at 1168
    Illegal BsaI site found at 1273
    Illegal BsaI site found at 2242


[edit]
Categories
Parameters
None