Part:BBa_K4382011

Xylanase Gene Construct

The gene construct contains 4 xylanase genes,a strong RBS(BBa_K4382009),and a signal peptide(BBa_K1074014).

Usage and Biology

The RBS is used to produce high levels of proteins and the signal peptide is used to secrete the enzymes.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 4128
  Illegal SpeI site found at 4099
  Illegal PstI site found at 200
  Illegal PstI site found at 3740
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 4128
  Illegal NheI site found at 205
  Illegal SpeI site found at 4099
  Illegal PstI site found at 200
  Illegal PstI site found at 3740
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 4128
  Illegal BglII site found at 3382
  Illegal BamHI site found at 929
  Illegal BamHI site found at 2179
  Illegal BamHI site found at 4164
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 4128
  Illegal SpeI site found at 4099
  Illegal PstI site found at 200
  Illegal PstI site found at 3740
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 4128
  Illegal SpeI site found at 4099
  Illegal PstI site found at 200
  Illegal PstI site found at 3740
  Illegal NgoMIV site found at 4627
  Illegal NgoMIV site found at 5041
  Illegal AgeI site found at 4612
  Illegal AgeI site found at 5470
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 609
  Illegal BsaI.rc site found at 1215
  Illegal BsaI.rc site found at 2915
  Illegal SapI.rc site found at 663