Part:BBa_K4382003

Beta-xylosidase (XynB)

It is used to degrade Xylanase

Usage and Biology

This gene, alongside Xylanase A (XynA) was discovered through screening of mutant lines of Bacillus subtilis. In this experiment, mutant lines of Bacillus subtilis 168 were created and were grown on plates with Xylan as the only carbon source.

Two types of Xylanase mutants were obtained - one which can’t grow in pure xylan and the other which grows very slowly. The gene in question is involved in the latter mutation. Xylanase B breaks down xylo-oligosaccharides produced from the breakdown of Xylanase A to form the monomeric units of xylan, i.e. xylose. In our project, xylose formation by breakdown of xylo-oligosaccharides shall be catalyzed by XynB. The optimal working temperature for xylanase B is 42 degrees celsius at a pH of 6.5.

References

Roncero, M. I. (1983, October). Genes controlling xylan utilization by Bacillus subtilis. Journal of Bacteriology, 156(1), 257–263. https://doi.org/10.1128/jb.156.1.257-263.1983

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 39
  Illegal BamHI site found at 1289
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 325