Part:BBa_K4380009
pACYC184 Rv primer
Introduction
Vilnius-Lithuania Igem 2022 project NanoFind used this part as a way mutate proteins on the bacterial surface. The team was working to create an easily accessible nanoplastic detection tool, using peptides, whose interaction with nanoplastic particles would lead to an easily interpretable response. The system itself focused on smaller protein molecules, peptides, which are modified to acquire the ability to connect to the surface of synthetic polymers – plastics. The detection system works when peptides and nanoplastic particles combine and form a sandwich complex - one nanoplastic particle is surrounded by two peptides, attached to their respective protein. The sandwich complex consisted of two main parts – one is a peptide bound to a fluorescent protein, another peptide is immobilized on a cellulose membrane by a cellulose binding domain. A bacterial cell surface display system was used as a novel way to expose the same peptides onto the bacterial membrane, allowing to try to amplify the signal and to find novel peptides by using a peptide evolution protocol. This part encodes a primer, used for amplifying pACYC184 plasmid backbone.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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