Intermediate

Part:BBa_K4378011

Designed by: Sigurður Smári Davíðsson   Group: iGEM22_Uppsala   (2022-10-06)


Overlap sequence for Gibson assembly

This is an overlap sequence we used for assembling tphA1 (BBa_4378006),tphA2 (BBa_4378007) and tphA3 (BBa_4378006) gene fragments. Note that the overlap is nonspecific to this gene and can be used in the Gibson assembly of other gene fragments.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None