Tag

Part:BBa_K4375014

Designed by: Zsóka Csorba   Group: iGEM22_ELTE   (2022-09-28)


Myc Tag

Myc Tag derived from c-Myc.



Usage and Biology

The Myc-tag, a commonly used tag in research and biological applications, is derived from the human c-myc oncogene (410-419 at the C-terminus). The Myc-tag is used in detecting the expression of recombinant proteins when it is fused to the protein of interest. Its sequence is EQKLISEEDL. It is commonly used in Western blotting, ELISA, protein purification ot co-immunoprecipitation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

„Myc-tag: An epitope tag for protein characterization, protein interaction analysis, and purification.†https://www.ptglab.com/news/blog/myc-tag-an-epitope-tag-for-protein-characterization-protein-interaction-analysis-and-purification/


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